Detection of infectious salmon anaemia virus by real-time RT–PCR
نویسندگان
چکیده
منابع مشابه
Genomic organization of infectious salmon anaemia virus.
The RNA genome segment order, nucleotide sequence and the putative encoded proteins were determined for infectious salmon anaemia virus (ISAV). Eight segments of genomic viral RNA between 1.0 and 2.4 kb in length were identified. RNA segments 1-6 each had a predicted single open reading frame encoding the P1, PB1, NP, P2, P3 and HA proteins, respectively. Segment 7 encoded the P4/P5 proteins an...
متن کاملRelative resistance of Pacific salmon to infectious salmon anaemia virus.
Infectious salmon anaemia (ISA) is a major disease of Atlantic salmon, Salmo salar, caused by an orthomyxovirus (ISAV). Increases in global aquaculture and the international movement of fish made it important to determine if Pacific salmon are at risk. Steelhead trout, Oncorhynchus mykiss, and chum, O. keta, Chinook, O. tshawytscha, coho, O. kisutch, and Atlantic salmon were injected intraperit...
متن کاملInfectious salmon anaemia virus (ISAV) mucosal infection in Atlantic salmon
All viruses infecting fish must cross the surface mucosal barrier to successfully enter a host. Infectious salmon anaemia virus (ISAV), the causative agent of the economically important infectious salmon anaemia (ISA) in Atlantic salmon, Salmo salar L., has been shown to use the gills as its entry point. However, other entry ports have not been investigated despite the expression of virus recep...
متن کاملTime course tissue distribution of infectious salmon anaemia virus in experimentally infected Atlantic salmon Salmo salar.
Atlantic salmon Salmo salar L. were injected intraperitoneally with infectious salmon anaemia virus (ISAV)-infective tissue homogenate to clarify the tissue distribution of ISAV in a time course study. Fish were sampled at 11 different intervals between 1 and 40 d post-infection (p.i.) and mid-kidney, head kidney, liver, spleen, intestine, gills, muscle and heart were tested for the presence of...
متن کاملCloning and identification of the infectious salmon anaemia virus haemagglutinin.
Infectious salmon anaemia virus (ISAV) is an orthomyxo-like virus that causes serious disease in Atlantic salmon (Salmo salar). Like the orthomyxoviruses, ISAV has been shown to possess haemagglutinin (HA) activity. This study presents the cloning, expression and identification of the ISAV HA gene, which was isolated from a cDNA library by immunoscreening. The HA gene contained an ISAV-specific...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2004
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2003.11.020